ACP
Effective spin probe for the EPR imaging and in vivo detection of intracellular
ROS (superoxide, peroxynitrite etc.). ACP can be easily protected with esterase
to yield cyclic hydroxylamine, which can be oxidized by reactive oxygen species to
yield ESR-detectable nitroxide radicals. The nitroxide radical is resistant to reduction
by vitamin C and thiols. Half-life time of nitroxide radical in biological environment
up to 4h. Samples in frozen condition (-80 °C or liquid nitrogen) can be stored
up to 6 months .

Rerferences

1. H. Yokoyama, et al.; In vivo EPR imaging by using an acyl-protected hydroxylamine to analyze intracerebral oxidative stress in rats after epileptic seizures: Magn. Reson. Imaging 18, 875 (Abstract)